Reference of 14215-68-0, Catalysts are substances that increase the reaction rate of a chemical reaction without being consumed in the process. 14215-68-0, Name is N-((2S,3R,4R,5R,6R)-2,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)acetamide, molecular formula is C8H15NO6. In a Article£¬once mentioned of 14215-68-0
Enzymatic Discrimination of 2-Acetamido-2-deoxy-D-mannopyranose-Containing Disaccharides Using beta-N-Acetylhexosaminidases
beta-N-Acetylhexosaminidase from Aspergillus oryzae selectively discriminates mixture of the disaccharides GlcNAcbeta(1?4)GlcNAc (1) and GlcNAcbeta(1?4)ManNAc (2). N,N?-Diacetylchitobiose (1) was selectively hydrolyzed by beta-N-acetylhexosaminidase, whereas its C-2 epimer (2) was completely resistant to the enzyme hydrolysis. Analogous discrimination was observed also with GalNAcbeta(1?4)GlcNAc (3) and GalNAcbeta(1? 4)ManNAc (4). beta-N-Acetylhexosaminidases from A. terreus, A. flavus, bovine kidney and bovine epididymis displayed the same selectivity, whereas the enzymes from A. sojae, A. tamarii, Penicillium brasilianum, P. oxalicum, P. funiculosum, P. multicolor, Talaromyces flavus and jack beans hydrolyzed both types of disaccharides. Molecular modelling of beta-N-acetylhexosaminidase from A. oryzae CCF 1066 and docking experiments with both types of disaccharides revealed that the ManNAc residue causes distortion of disaccharide molecule resulting in a steric conflict with a Trp482 that causes diminished stabilization of the oxazolinium transition state by extending the distance of Asp345 in the active site. Both ManNAc-containing disaccharides 2 and 4 dock with similar steric energies into the active site but without cleaving and also without notable inhibition. This novel phenomenon enables also the preparative production of both disaccharides 2 and 4 starting from N, N?-diacetylchitobiose (1) or GalNAcbeta(1? 4)GlcNAc (3) followed by Lobry de Bruyn-Albreda van Ekenstein C-2 epimerization catalyzed by Ca(OH)2. The resultant mixture of the respective epimers 1, 2 or 3, 4 that is hardly separable by, e.g., analytical HPLC can be treated with the beta-N-acetylhexosaminidase from A. oryzae and the mixture of monosaccharides and target disaccharide can be easily separated using gel filtration.
The proportionality constant is the rate constant for the particular unimolecular reaction. the reaction rate is directly proportional to the concentration of the reactant. I hope my blog about 14215-68-0 is helpful to your research., Reference of 14215-68-0
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Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics