Chemical research careers are more diverse than they might first appear, as there are many different reasons to conduct research and many possible environments. Introducing a new discovery about 14215-68-0, Name is N-((2S,3R,4R,5R,6R)-2,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)acetamide, 14215-68-0.
beta-D-Galactosidase was purified 115-fold from a saline extract of papaya seeds by fractionation with ammonium sulfate, DEAE-Sephadex chromatography and gel-filtration on Sephadex G-75, G-150, and G-100.The purified beta-D-galactosidase (MW, 56,000 daltons) had an isoelectric point (pI) at pH 8,4 and the optimal pH for its activity was 3.5 to 4.5.The enzyme activity was inhibited by Cu(2+), Ag(1+), Hg(2+), Pb(2+), NaAsO2 and p-chloromercuribenzoate at concentrations of 1E-3 M.Among the various mono- and oligosaccharides tested, D-galactose, D-galacturonic acid, D-galactono-gamma-lactone and melibiose significantly inhibited the enzyme activities at concentrations of 2E-3 to 1E-2 M.The purified enzyme hydrolyzed p-nitrophenyl beta-D-galactoside (Km = 1.0E-3 M), methyl beta-D-galactoside (Km = 1.6E-2 M), aminoethyl beta-D-galactoside (Km = 3.3E-2 M) and lactose (Km = 9.1E-2 M). beta-(1–>3)-Linked galactotetraosyl-erythritol and asialo-glycopeptide isolated from fetuin were also hydrolyzed to the extent of 78 and 75percent, respectively, on the basis of their galactose contents. alpha-D-Mannosidase from papaya seeds was also purified 130-fold by ammonium sulfate fractionation, DEAE-Sephdex chromatography, gel-filtration on Sephadex G-150 and hydroxylapatite chromatography.The purified enzyme (MW, 156,000 daltons), consisting of two subunits (78,000 x 2), was inhibited by Hg(2+), Ag(1+), Cu(2+), p-chloromercuribenzoate, D-glucose, D-glucosamine and D-mannose at concentration of 1E-3 to 1E-2 M.The alpha-D-mannosidase hydrolyzed p-nitrophenyl alpha-D-mannoside (Km = 5.6E-3 M), methyl alpha-D-mannoside (Km = 2.8E-2 M), alpha-D-mannosyl-D-mannitol (Km = 2.2E-2 M), alpha (1–>2)-linked D-mannobiosyl-D-mannitol (Km = 6.3E-3 M) and D-mannotriosyl-D-mannitol (Km = 5.3E-3 M).
Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law.14215-68-0. You can get involved in discussing the latest developments in this exciting area about 14215-68-0
Reference：
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics