Application of a robust microplate assay to determine induced β-1,3-glucanase and chitinase activity in the cotton plant was written by Khan, Muhammad Fahad;Umar, Ummad-ud-din. And the article was included in BioTechniques in 2021.Related Products of 9008-22-4 This article mentions the following:
Resistance is induced in cotton plants as the result of either viral infection or exogenous application of elicitors. Induced resistance can be evaluated by determining the production of β-1,3-glucanase and chitinase in plants as a biochem. parameter. The assays being used for the determination of chitinase and β-1,3-glucanase activity are laborious and not cost-effective, as the reducing sugars produced by the substrates colloidal chitin and laminarin are very expensive. The concentration of both substrates was standardized and reduced to 0.25% from 4% in a modified microplate assay, which appeared to be more effective. The amount of β-1,3-glucanase and chitinase produced was significant and determined by the new modified assay. The sensitivity of the microplate assay was significantly raised approx. one- to twofold. The existing procedure for determining resistance induced in cotton plants by viruses and elicitors is time-consuming and expensive. However, this resistance can be determined by β-1,3-glucanase and chitinase activity through a modified, cost-effective and robust technique. Here the authors present a 96-well polystyrene microplate assay, instead of a quartz cuvette assay, in which a decreased amount of costly substrates was used and a large number of samples were processed in a short time. In the experiment, the researchers used many compounds, for example, Laminarin (cas: 9008-22-4Related Products of 9008-22-4).
Laminarin (cas: 9008-22-4) belongs to tetrahydropyran derivatives. Tetrahydropyrans are also used as important solvents, as chemical intermediate and as monomer for ring-opening polymerization. 2-(Arylmethylene)cyclopropylcarbinols could be converted to the corresponding tetrahydropyrans stereoselectively in the presence of Brønsted acids under mild conditions. A plausible Prins-type reaction mechanism has been proposed.Related Products of 9008-22-4
Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics