Day: February 22, 2023

Defining genome architecture at base-pair resolution was written by Hua, Peng;Badat, Mohsin;Hanssen, Lars L. P.;Hentges, Lance D.;Crump, Nicholas;Downes, Damien J.;Jeziorska, Danuta M.;Oudelaar, A. Marieke;Schwessinger, Ron;Taylor, Stephen;Milne, Thomas A.;Hughes, Jim R.;Higgs, Doug R.;Davies, James O. J.. And the article was included in Nature (London, United Kingdom) in 2021.SDS of cas: 11024-24-1 This article mentions the following:

In higher eukaryotes, many genes are regulated by enhancers that are 104-106 base pairs (bp) away from the promoter. Enhancers contain transcription-factor-binding sites (which are typically around 7-22 bp), and phys. contact between the promoters and enhancers is thought to be required to modulate gene expression. Although chromatin architecture has been mapped extensively at resolutions of 1 kilobase and above; it has not been possible to define phys. contacts at the scale of the proteins that determine gene expression. Here we define these interactions in detail using a chromosome conformation capture method (Micro-Capture-C) that enables the phys. contacts between different classes of regulatory elements to be determined at base-pair resolution We find that highly punctate contacts occur between enhancers, promoters and CCCTC-binding factor (CTCF) sites and we show that transcription factors have an important role in the maintenance of the contacts between enhancers and promoters. Our data show that interactions between CTCF sites are increased when active promoters and enhancers are located within the intervening chromatin. This supports a model in which chromatin loop extrusion is dependent on cohesin loading at active promoters and enhancers, which explains the formation of tissue-specific chromatin domains without changes in CTCF binding. In the experiment, the researchers used many compounds, for example, Digitonin (cas: 11024-24-1SDS of cas: 11024-24-1).

Digitonin (cas: 11024-24-1) belongs to tetrahydropyran derivatives. Tetrahydropyrans are also used as important solvents, as chemical intermediate and as monomer for ring-opening polymerization. Pyran derivatives such as pyran flavonoids are biologically important. Monosaccharides containing six-membered rings are called pyranose.SDS of cas: 11024-24-1

Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics

Phenylphosphinic acid-promoted addition of isocyanide to 1-methoxyisochroman derivatives was written by Soeta, Takahiro;Matsuzaki, Syunsuke;Ukaji, Yutaka. And the article was included in Heterocycles in 2018.Formula: C6H12O2 This article mentions the following:

A synthetic method for preparation of isochroman-1-carboxylamides I [R¹ = H, 5-Me, 7-Br, etc.; R² = t-Bu, Ph, Bn, etc.] was developed in good to high yields by addition of isocyanides to 1-methoxyisochroman derivatives in the presence of phosphinic acid. A wide range of 1-methoxyisochroman derivatives and isocyanides were suitable for this Passerini-type reaction. In the experiment, the researchers used many compounds, for example, 2-Methoxytetrahydro-2H-pyran (cas: 6581-66-4Formula: C6H12O2).

2-Methoxytetrahydro-2H-pyran (cas: 6581-66-4) belongs to tetrahydropyran derivatives. Numerous natural products have tetrahydropyran skeleton as the building block for designing new natural products and their derivatives e.g. aplysiatoxins, avermectins, oscillatoxins, talaromycins, latrunculins and acutiphycins. The reaction of tertiary 1,4- and 1,5-diols with cerium ammonium nitrate at room temperature gives tetrahydrofuran and tetrahydropyran derivatives in high yield and stereoselectivity. Various fragrant compounds have been synthesized using this method.Formula: C6H12O2

Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics