Vitamin E performs antioxidant effect via PAP retrograde signaling pathway in Nile tilapia (Oreochromis niloticus) was written by Xu, Minjun;Ye, Jiawei;Wang, Yujie;Chu, Kejie;Pere, Maxime;Xu, Minjie;Tang, Xuelian;Fu, Jinghua. And the article was included in Fish & Shellfish Immunology in 2022.Quality Control of Dextrin This article mentions the following:
PAP (3鈥?phosphoadenosine 5鈥?phosphate) is a ubiquitous phosphoric acid and a natural inhibitor of the XRN (5鈥?3鈥瞖xoribonuclease) family. It was proved to enter the nucleus through the retrograde signaling pathway and inhibit XRN2 to prevent the degradation of miRNA precursors, thus promoting the anti-oxidation miRNA level in Arabidopsis thaliana. Vitamin E (tocopherol) was proved to promote the accumulation of PAP in the plant, which facilitates PAP into the nucleus to accomplish its antioxidant function. However, the relationship between VE and PAP in animals is unclear. To identify the relationship between VE and PAP and to uncover the function of PAP in fish, we investigated the performance of VE and PAP in Nile tilapia by comparing the antioxidant indicators (SOD, GSH-Px, and CAT), the Keap1-Nrf2 signaling pathway, and the miRNA expression profiles. Results showed that the antioxidant effect of VE and PAP showed similar character either in tilapia liver or in serum: the activities of GSH-Px and CAT of both groups were significantly increased (P < 0.05); the SOD activity of the VE group was significantly increased (P > 0.05), and although the result of the PAP group was not so significant (P < 0.05), PAP improved the SOD level, too. The two groups also showed similar character in the tilapia liver; both did not significantly increase the liver 未-VE content (P > 0.05). However, VE significantly increased the content of 伪-VE and 纬-VE (P > 0.05), while the PAP group was insignificant (P >0.05). Feed with VE and i.p. injection of PAPs reagent both increased the PAP content in the liver of tilapia, and the effect of the VE group was more significant (P <0.05) than that of the PAP group (P < 0.05). Both groups reduced the expression of Keap1 and Cullin3 genes and improved the level of HO-1 gene expression, with the improved miRNA level of Nrf2. As a logical result, they decreased the expression of XRN1 and XRN2. By profile sequencing, we further identified some antioxidant closely related miRNAs shared in the VE and PAP groups, including miR-30, miR-24, miR-19b, and miR-100. By comparing the regulating mechanism of VE and PAP of feed supply and i.p. injection, we proved that VE and PAP were closely related in fish; VE promoted the gathering of PAP. The latter retrograded into the nucleus of the fish liver to inhibit the expression of XRN genes and to up-regulate antioxidant miRNA levels as it does in plants. Only the PAP can accomplish the antioxidant activities, while VE promotes the process. Our study laid the foundation for the application of PAP as a new antioxidant agent in fish farming and benefit a further understanding of the VE antioxidant function in fish. In the experiment, the researchers used many compounds, for example, Dextrin (cas: 9004-53-9Quality Control of Dextrin).
Dextrin (cas: 9004-53-9) belongs to tetrahydropyran derivatives. Tetrahydropyran is an important raw material and intermediate used in Organic Synthesis, Pharmaceuticals, Agrochemicals and dyestuff. 2-(Arylmethylene)cyclopropylcarbinols could be converted to the corresponding tetrahydropyrans stereoselectively in the presence of Br酶nsted acids under mild conditions. A plausible Prins-type reaction mechanism has been proposed.Quality Control of Dextrin
Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics