Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility was written by Futamata, Haon;Fukuda, Masahiro;Umeda, Rie;Yamashita, Keitaro;Tomita, Atsuhiro;Takahashi, Satoe;Shikakura, Takafumi;Hayashi, Shigehiko;Kusakizako, Tsukasa;Nishizawa, Tomohiro;Homma, Kazuaki;Nureki, Osamu. And the article was included in Nature Communications in 2022.Name: Digitonin This article mentions the following:
Outer hair cell elecromotility, driven by prestin, is essential for mammalian cochlear amplification. Here, we report the cryo-EM structures of thermostabilized prestin (PresTS), complexed with chloride, sulfate, or salicylate at 3.52-3.63 脜 resolutions The central pos.-charged cavity allows flexible binding of various anion species, which likely accounts for the known distinct modulations of nonlinear capacitance (NLC) by different anions. Comparisons of these PresTS structures with recent prestin structures suggest rigid-body movement between the core and gate domains, and provide mechanistic insights into prestin inhibition by salicylate. Mutations at the dimeric interface severely diminished NLC, suggesting that stabilization of the gate domain facilitates core domain movement, thereby contributing to the expression of NLC. These findings advance our understanding of the mol. mechanism underlying mammalian cochlear amplification. In the experiment, the researchers used many compounds, for example, Digitonin (cas: 11024-24-1Name: Digitonin).
Digitonin (cas: 11024-24-1) belongs to tetrahydropyran derivatives. Tetrahydropyrans are also used as important solvents, as chemical intermediate and as monomer for ring-opening polymerization. 2-(Arylmethylene)cyclopropylcarbinols could be converted to the corresponding tetrahydropyrans stereoselectively in the presence of Br酶nsted acids under mild conditions. A plausible Prins-type reaction mechanism has been proposed.Name: Digitonin
Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics