Complementary developing solvents for simpler and more powerful routine analysis by high-performance thin-layer chromatography was written by Do, Thi Kieu Tien;Schmid, Marco;Trettin, Ilona;Hanni, Mona;Reich, Eike. And the article was included in Journal of Planar Chromatography–Modern TLC in 2022.Computed Properties of C16H22O10 This article mentions the following:
Because the separation power of an high-performance thin-layer chromatog. (HPTLC) plate is difficult to increase, method development in HPTLC commonly focuses on the optimization of resolution between selected zones and has, over the years, led to a plethora of similar developing solvents containing the same components in different ratios. Laboratories performing routine HPTLC anal. based on monographs prescribed by pharmacopoeias, thus, have to maintain numerous different methods, solvents, and standards In order to simplify and harmonize the routine anal. of many diverse samples, this paper presents an innovative approach based on the parallel use of three complementary developing solvents (CDS) featuring different polarity and selectivity. The graphical or computational combination of the resulting three fingerprints expands the chromatog. profile of complex samples. The power of the concept is illustrated with HPTLC fingerprints for identification of some herbal drugs, herbal products, and a poly-herbal formulation. Furthermore, the CDS is able to differentiate and identify individual compounds Thirty-one substances, including iridoids, coumarins and carbohydrates were exemplarily analyzed. All substances migrate to RF values between 0.2 and 0.8 in at least one of the developing solvents, and discrimination of the substance classes is well accomplished. As central element of an advanced HPTLC protocol for routine application and combined with further multidimensional data anal., this CDS could provide the basis for computational methods that are able to evaluate large sets of HPTLC data in the near future. In the experiment, the researchers used many compounds, for example, (4aR,5R,6S)-4a-Hydroxy-6-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-5-vinyl-4,4a,5,6-tetrahydropyrano[3,4-c]pyran-1(3H)-one (cas: 17388-39-5Computed Properties of C16H22O10).
(4aR,5R,6S)-4a-Hydroxy-6-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-5-vinyl-4,4a,5,6-tetrahydropyrano[3,4-c]pyran-1(3H)-one (cas: 17388-39-5) belongs to tetrahydropyran derivatives. Tetrahydropyrans are useful synthons for biologically important compounds. 2-(Arylmethylene)cyclopropylcarbinols could be converted to the corresponding tetrahydropyrans stereoselectively in the presence of Br酶nsted acids under mild conditions. A plausible Prins-type reaction mechanism has been proposed.Computed Properties of C16H22O10
Referemce:
Tetrahydropyran – Wikipedia,
Tetrahydropyran – an overview | ScienceDirect Topics